Phloem Loading of Sucrose

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Sucrose transporter1 functions in phloem loading in maize leaves

In most plants, sucrose is exported from source leaves to carbon-importing sink tissues to sustain their growth and metabolism. Apoplastic phloem-loading species require sucrose transporters (SUTs) to transport sucrose into the phloem. In many dicot plants, genetic and biochemical evidence has established that SUT1-type proteins function in phloem loading. However, the role of SUT1 in phloem lo...

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Companion-Cell Specific Localization of Sucrose Synthase in Zones of Phloem Loading and Unloading.

An immunohistochemical approach was used in maize (Zea mays) and citrus (Citrus paradisi) to address the previously noted association between sucrose synthase and vascular bundles and to determine the localization of the low but detectable levels of sucrose synthase that remain in leaves after the import-export transition. Sucrose synthase protein was immunolocalized at the light microscope lev...

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Energy-dependent Loading of Amino Acids and Sucrose into the Phloem of Soybean.

Radioactive sucrose, l-leucine, l-glutamate, and gamma-aminobutyrate were applied exogenously to abraded areas of soybean leaves. The three amino acids were translocated with similar velocities and mass transfer rates on a molar basis, although they were metabolized differently in the sink tissue. The concentration dependence of leucine translocation showed a triphasic saturation response, whil...

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Phloem loading in squash.

Squash (Cucurbita pepo L. var. melopepo torticalis, Bailey) leaves were supplied with (14)C-sucrose, then specific radioactivities of the glucose and galactose moieties of translocated stachyose were determined. In every case, the specific radioactivity of the galactose moiety was greater than that of the glucose moiety. It is concluded that the stachyose was not synthesized at either the phloe...

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Phloem Loading by the PmSUC2 Sucrose Carrier from Plantago major Occurs into Companion Cells.

High levels of mRNA for the sucrose-H+ symporter PmSUC2 have been found in the vascular bundles of petioles from Plantago major. The possible role of PmSUC2 in phloem loading was studied with antiserum raised against the recombinant PmSUC2 protein. This antiserum labeled a single 35-kD protein band in detergent extracts of P. major vascular bundles. It showed no cross-reaction with the P. major...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1977

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.59.4.750